39450-01-6 Proteinase K

CAS:39450-01-6
MF:C29H27N2O12P
MW:626.504641
EINECS:254-457-8
MDL No.:MFCD00132129

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Description

Specification:

Appearance	White loose powder
Activity	≥30U/mg
Specific activity	≥40U/mgP
DNase activity	Should not be detected
RNase activity	Should not be detected
pH	5.0-7.0
Protein content	≥95%

Storage: STORE IN A COOL, DRY AND LIGHTLESS PLACE, 2-8℃.

Application:

Protease K is widely used in fields such as molecular biology, biochemistry, and biochemistry. Protease K is a powerful proteolytic enzyme isolated from Candida albicans, with high specific activity, and is a key reagent for DNA extraction. This enzyme is active in a wide pH range (4-12.5) and high temperature (50-70 ° C), and is used for the isolation of plasmids or genomic DNA and RNA. In DNA extraction, the main function is enzymatic hydrolysis of histones bound to nucleic acids, allowing DNA to be free in solution. Subsequently, different methods are used for extraction to remove impurities and collect DNA. Neither chelating agents such as EDTA nor descaling agents such as SDS can inactivate it

Synonyms:

Protease K
proteinase K crude
Recombinant Proteinase K
PROTEINASE K, LYOPHILIZED
PROTEINASE K, IMMOBILIZED
Tritirachium album proteinase K
proteinase K aseptically filled
proteinase K for tritirachium album
proteinase K from tritrachium album

Properties

Safety Information

Statements

solubility H2O: 10 mg/mL, slightly hazy, brown-yellow

pH 7.5～12

Water Solubility Miscible with water.

Symbol(GHS)

GHS08

Signal word Danger

Hazard statements H315-H319-H334

Precautionary statements P261-P264-P280-P284-P302+P352-P305+P351+P338

Hazard Codes Xn